Operative Techniques in Orthopaedics
Volume 20, Issue 2 , Pages 106-109 , June 2010

Cell Traction Forces (CTFs) and CTF Microscopy Applications in Musculoskeletal Research

  • James H.-C. Wang, PhD

      Affiliations

    • Corresponding Author InformationAddress reprint requests to James H.-C. Wang, PhD, Departments of Orthopaedic Surgery, Bioengineering, Mechanical Engineering and Materials Science, Physical Medicine and Rehabilitation, MechanoBiology Laboratory, University of Pittsburgh, 210 Lothrop St, BST, E1640, Pittsburgh, PA

References 

  1. Wang JH, Thampatty BP. An introductory review of cell mechanobiology. Biomech Model Mechanobiol. 2006;5:1–16
  2. Wang JH, Lin JS. Cell traction force and measurement methods. Biomech Model Mechanobiol. 2007;6:361–371
  3. Ingber DE. Mechanosensation through integrins: Cells act locally but think globally. Proc Natl Acad Sci USA. 2003;100:1472–1474
  4. Ingber DE. Tensegrity: The architectural basis of cellular mechanotransduction. Annu Rev Physiol. 1997;59:575–599
  5. Harris AK, Stopak D, Wild P. Fibroblast traction as a mechanism for collagen morphogenesis. Nature. 1981;290:249–251
  6. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA. 1979;76:1274–1278
  7. Moon AG, Tranquillo RT. The fibroblast-populated collagen microsphere assay of cell traction force—Part 1 (Continuum model). AIChE J. 1993;39:163–177
  8. Delvoye P, Wiliquet P, Leveque JL, et al. Measurement of mechanical forces generated by skin fibroblasts embedded in a three-dimensional collagen gel. J Invest Dermatol. 1991;97:898–902
  9. Campbell BH, Clark WW, Wang JH. A multi-station culture force monitor system to study cellular contractility. J Biomech. 2003;36:137–140
  10. Harris AK, Wild P, Stopak D. Silicone rubber substrata: A new wrinkle in the study of cell locomotion. Science. 1980;208:177–179
  11. Burton K, Taylor DL. Traction forces of cytokinesis measured with optically modified elastic substrata. Nature. 1997;385:450–454
  12. du Roure O, Saez A, Buguin A, et al. Force mapping in epithelial cell migration. Proc Natl Acad Sci USA. 2005;102:2390–2395
  13. Tan JL, Tien J, Pirone DM, et al. Cells lying on a bed of microneedles: An approach to isolate mechanical force. Proc Natl Acad Sci USA. 2003;100:1484–1489
  14. Li B, Xie L, Starr ZC, et al. Development of micropost force sensor array with culture experiments for determination of cell traction forces. Cell Motil Cytoskeleton. 2007;64:509–518
  15. Pelham RJ, Wang Y. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci USA. 1997;94:13661–13665
  16. Butler JP, Tolic-Norrelykke IM, Fabry B, et al. Traction fields, moments, and strain energy that cells exert on their surroundings. Am J Physiol Cell Physiol. 2002;282:C595–C605
  17. Yang Z, Lin JS, Chen J, et al. Determining substrate displacement and cell traction fields—A new approach. J Theor Biol. 2006;242:607–616
  18. In:  Kwok-Sui L,  Qin L,  Cheung WH editor. A practical manual for musculoskeletal research. Singapore: World Scientific Publishing; 2008;
  19. Li B, Lin M, Tang Y, et al. A novel functional assessment of the differentiation of micropatterned muscle cell. J Biomech. 2008;41:3349–3353
  20. Anderson S, DiCesare L, Tan I, et al. Rho-mediated assembly of stress fibers is differentially regulated in corneal fibroblasts and myofibroblasts. Exp Cell Res. 2004;298:574–583
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 Supported by NIH grants AR049921 and AR049921-S2 (J.H.-C.W.).

PII: S1048-6666(09)00147-5

doi: 10.1053/j.oto.2009.10.007

Operative Techniques in Orthopaedics
Volume 20, Issue 2 , Pages 106-109 , June 2010

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